Korewharewha Whakakitenga PCR Ine 96 tauira
Hei whiriwhiringa e tika ana mo te tātari ine ine o te koiora irapoi, kua whakamahia whanuitia te punaha PCR i roto i nga momo mara penei i te rangahau putaiao, te rapunga haumanu me te tātaritanga, te kounga me te whakamatautau haumaru, me nga tono mo te tirotiro.
Pūnaha PCR Wā-tūturu
Tika 96
Ngā āhuatanga
• Tae atu ki te 6 nga hongere rapu rewharewha ka taea te PCR maha.
• He pai te whakaiti i te korero whakawhiti korero maha-tae me te paanga taha, kaore he whakatika ROX hei whakaiti i te tauira me te whakamahi reagent
• Tikanga matawai hou me te hangarau wehewehe tohu kua whakatauhia mo te wa ki te whakapai ake i te arokitenga
• Hangarau utu pamahana tapa motuhake hei whakaiti i te "painga taha"
• pūmanawa ratarata-kaiwhakamahi
• Ko te hangarau hou me te rama LED-roa-roa e whakarato ana i nga hua pono
Tawhā Hangarau
| Pūnaha whakahaere pāmahana | |
| Te kaha tauira | 96 |
| Rōrahi tauhohenga | 10-50 μl |
| Hangarau huringa wera | Peltier |
| Max.Reiti Whakawera/Matao | 6.0° C/s |
| Te awhe pāmahana whakawera | 4 – 100 °C |
| Te tika o te pāmahana | ± 0.2°C |
| Te ōritetanga o te pāmahana | ±0.2 ℃ @60 ℃ , ± 0.3 ℃ @95 ℃ |
| Awhe tautuhinga rōnaki pāmahana | 30–100°C |
| Awhe tautuhinga rerekētanga rōnaki pāmahana | 1 – 36°C |
| Pūnaha rapu | |
| Te puna marama whakahihiri | 4/6 monochrome nga rama rama teitei |
| Pūrere rapu | PMT |
| Aratau rapunga | Hangarau wehewehe tohu kua whakatauhia e te waa |
| Te awhe roangaru whakahihiri/kitenga | 455-650nm/510-715nm |
| Ko nga hongere rewharewha | 4/6 hongere |
| Waikano tautoko | FAM/SYBR Green, VIC/JOE/HEX/TET, ABY/NED/TAMRA/Cy3, JUN, ROX/Texas Whero, Mustang Purple, Cy5/LIZ |
| Te tairongo | Ira kape kotahi |
| Whakatau | 1.33 takai rerekeetanga tau kape ka taea te wehewehe ki te qPCR kotahi-plex |
| Awhe hihiko | 10 ota kape nui |
