Ko te riaka wai he riaka kua whakatipuria ki te reo tikanga wai.Kei a ia nga painga o te huringa whakaputa riaka poto, te tau huakita rite, te inoculation watea, e tika ana mo te hanga wheketere, me era atu.Kua powhiritia e te nuinga o nga kaiahuwhenua.Rooru pūtau （https://www.luoron.com/plastic-erlenmeyer-flask-with-vent-cap-2-product/） he momo rauemi pau e hiahiatia ana i roto i te ahurea o nga riaka wai.Ko nga mea e whai ake nei me aro ki te ahurea:

1.Preparation o te reo tikanga rite ki te tātai o ngā wāhanga, ki te ipu, memeha ki te wai, wehea ki ipu tapatoru, te tikanga 250 ~ 300mL pūtau ruru pounamu 50mL tikanga tikanga,500mL pounamu tapatoru  （https://www.luoron.com/plastic-erlenmeyer-flask-with-vent-cap-2-product/）100mL tikanga tikanga, mono miro mono, me te niupepa takai;Ka taea hoki te hanga i nga paparanga 8 o te kakano 8cm×8cm te hiri pounamu, kei waho me te hiri pepa parauri.
2. Ko te tikanga whakamaoritanga Autoclave te tikanga mo te whakahoromata, me 121 ℃ me te 30min.Tangohia ki waho ka whakamatao ki te 30 ℃.Hoatu ki roto i te rūma pokekore, i te pouaka werohanga ranei hei whakamahi.
3. E ai ki nga whakaritenga mahi aseptic, ka taea te hono atu i ia pounamu ki te 2 ~ 3cm2 riaka hiku, a ka taea te hono atu ia riaka ki te 4 ~ 5 ipu.He pai ake te kawe mai i etahi reo me nga riipene taapiri, kia rewa ai i runga i te mata o te reo.Whai muri i te werohanga, uhia te waha o te pounamu ki te kakano i kuia i te tuatahi, ka herea ki te aho.
4. Ka taea te whakanoho i te pounamu riaka kua werohia ki roto i te moenga wiri mo te ahurea, ka taea ranei te whakanoho ki roto i te mahana tonu o 24 ~ 26 ℃ mo te 48h, katahi ka taea te kawe i te ahurea oscillated i muri i te toronga o te mycelium aerogenic ki te reo ahurea. .Ko te auau oscillating o reciprocating wiri ko 80 ~ 120r/min, me e o wiri hurihuri ko 150 ~ 220r/min.3 ~ 4d ka taea te whakatipu i roto i te wiri.I te mutunga o te ahurea, na te rerekee o nga taumahatanga, he rereke nga tae o te momo tikanga, penei i te harore tio, te reo harore enoki he kowhai marama;Ko te harore, ericius erinaceus culture medium he parauri whero, me te kakara o te harore;He parauri, he matotoru, he reka te tikanga o te ahurea harore.Ka taea te whakamahi i te reo ahurea mo te whakaputa me te whakanui ake ranei i te ahurea ka tae te taumaha maroke o te mycelium ki te 10g/L me te diameter o nga pire mycelium he 1 ~ 2mm.
 
5. Te hanga riaka wai tuarua tuarua te riaka wai tikanga hanga reo o te taumata rite o te riaka wai, me nui ake te ipu ahurea, kaua e neke ake i te 3500mL te kaha.Whai muri i te whakahoroa me te whakamatao, ka kuhuhia nga riaka wai tuatahi kua fermented ki roto5L wiriwiri pūtau（https://www.luoron.com/3l5l-high-efficiency-erlenmeyer-flask-product/）i te ōwehenga o te 5%-10% me te whakatipu i runga i te wiri i te tere puhoi.Whai muri i etahi wa o te ahurea oscillating, ka taea te whiwhi i nga riu wai me te tohatoha rite o nga microspheres me te wai FERMENTATION maamaa.
Hei whakarāpopototanga, i te wa e whakamahi ana i te wiriwiri pūtau ki te whakatipu i nga riaka wai, ka mahi i runga i nga tikanga o runga ake nei, me te aro ki te whakahaere i te pāmahana, te wiri me etahi atu mea i roto i te mahi hei whakarite i te paanga o te ahurea.
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